HLA-DRAMajor Histocompatibility Complex, Class II, DR Alpha
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Similarly, we found the postoperative production of HLA-DRA mRNA in both groups to be reduced compared with preoperative values, a finding that reflects the situation before and after an inflammatory stimulus.
To control the extraction efficiency of DNA from stool, two capture oligonucleotides from HLA-DRA were designed as perfect-match (PM) and mismatch (MM) probes.
Significant inhibition of the PCR reaction by remaining impurities was not observed, as indicated by our successful amplification of HLA-DRA.
A missing amplification of the internal control HLA-DRA was taken as an indication that not enough human DNA was extracted or originally present in the stool sample or that PCR-inhibiting substances were present.