Mitochondrial sirtuin 3 (SIRT3), a nicotinamide adenine dinucleotide-dependent histone deacetylase, is associated with the antioxidation of CR and can improve the activity of HMGCS2 via deacetylation.
After extraction and verification, plasmid pcDNA HMGCS2 transfection was conducted using Lipofectamine 3000 transfection reagent according to the manufacturer's instructions.
GTPs Upregulate the Expression of HMGCS2 and Its Catalyst in the Renal Cortexes of HFD-Treated Rats.
Furthermore, HMGCS2 activity can be upregulated by SIRT3.
HEK293 Cells Transfected with the Plasmid pcDNA HMGCS2 Show Lower Levels of 4-HNE and Acetylated MnSOD.
These were consistent with our previous study that the expression of HMGCS2 could be upregulated by GTPs through PPAR-[alpha] pathway.
It has been reported that HMGCS2 can be deacetylated by SIRT3, a member of the class III histone deacetylases (sirtuins), subsequently increasing ketogenesis [40,41].
These data indicate that HMGCS2 could increase SIRT3 activity against oxidative stress.
HMGCS2: Mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase 2 [beta]HB: [beta]-Hydroxybutyrate HDAC: Histone deacetylase SIRT3: Sirtuin 3 MnSOD: Manganese superoxide dismutase FOXO3a: Forkhead box O3a GTPs: Green tea polyphenols CON: Control HFD: High-fat diet Nampt: Nicotinamide phosphoribosyltransferase 4-HNE: 4-Hydroxy-2-nonenal KB: Ketone body CR: Calorie restriction ROS: Reactive oxygen species NAG: N-Acetyl-[beta]-D-glucosaminidase TC: Total cholesterol TG: Triglycerides HDL-C: High-density lipoprotein-cholesterol LDL-C: Low-density lipoprotein-cholesterol.