Por otro lado, la adicion de un grupo metilo mediante la histonametiltransferasa (HMT
, EC 126.96.36.199) puede ocurrir en varios residuos basicos de las histonas.
Methylation is orchestrated by HMTs
responsible for the arginine methylation are called protein arginine methyltransferases (PRMTs), and 11 isoforms, divided into 3 groups, type I, II, and III, exist in mammals (36).
Among them, a great majority are known inhibitors of HDACs, HMTs
, and/or DNMTs, which is in contrast with the outcome of an earlier human HTS, where a number of non-HDAC inhibitors were identified , which may be due to a difference in the selection of compound libraries.
In both in vitro and in vivo investigations related to diabetes, it has been demonstrated that histone lysine methylation and acetylation patterns changed, along with the recruitment of HATs/HDACs or HMTs
at gene promoters [6, 48, 56, 57].
Consequently, this activates a pool of inflammasomes (AIM2, ACS, NLRP3, and IFI16) and hepatoepigenetics via epigenetic regulators (DNMTs, TETs, HMTs
, and HDMTs) that trigger aberrant transcriptional activities of hepatitis B x (HBx) protein and altered gene transcription leading to hepatocarcinogenesis.
In Type 1 direct pathway, IM directly exerts an effect on the epigenetic enzymes such as DNMTs, HDACs, HATs, HMTs
, and HDMs, such that there is an altered bioavailability of these enzymes in the cell.
G9a, GLP, and suppressor of variegation 3-9 homolog 1 (SUV39H1) are another group of HMTs
responsible for H3K9 methylation in association with p53.
Custom assay development to adapt this assay format to other HMTs
is also available through Cayman's epigenetic screening and profiling service.
TABLE 1: Different classes of histone methyl transferases (HMTs
The HOXA9 gene is primarily regulated by HMTs
and encodes a DNA-binding transcription factor which contributes to regulating gene expression, morphogenesis and differentiation.
A new report from BCC Research says increased R&D spending for development of epigenetic tools and assays for Histone Methyltransferases (HMT
) research, HMTs
use as biomarkers and targets for diagnostics development and the drug discovery sector, will "drive tremendous growth" in this market for the foreseeable future.
The activities of enzymes involved in epigenetic modifications, such as DNA methyltransferases (DNMTs), histone acetyltransferases (HATs), HDACs, histone methyltransferases (HMTs
), and histone demethylases (HDMs), are regulated, in part, by the concentrations of their required substrates and cofactors (Lee and Workman 2007).