99) of CT-DNA alkylated with MAM or HN2, respectively.
Primary cerebellar neuronal cultures treated for 24 hr with MAM or HN2 were examined for DNA fragmentation using TUNEL with the NeuroTacs staining kit according to the manufacturer's instructions (Trevigen, Gaithersburg, MD).
0 [micro]M HN2 using Tri-Reagent (Molecular Research Corp.
In the present study, our goal was to determine the relationship between the sensitivity of immature postmitotic neurons to MAM or HN2 and their ability to damage DNA.
Additional studies were conducted to determine if the increased sensitivity of neurons to MAM and HN2 was due to their genotoxic (i.
0 [micro]M HN2 for 24 hr and examined total RNA for gene expression changes using high-density microarrays.