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We coated 96-well plates with fibronectin as previously described and seeded 5 x 104 SH-SY5Y cells or 2 x 104 hNSCs per well.
Human neural stem cells (hNSC; H9 hESC-Derived) and culture medium were obtained from Life Technologies.
(b) Hospital Nossa Senhora da Conceicao (HNSC), Porto Alegre, RS, Brazil
In this work, four TCGA datasets, BRCA, HNSC, KIRC, and THCA, were applied to our method.
There may also be a slight change in the ratio of Democrats to Republicans on the HNSC because of the narrowed House margin.
For growth assay, human neural stem cells (hNSCs) were seeded in 24 well plates at 5 x [10.sup.4] per well and treated with the control vehicle phosphate-buffered saline (PBS) or human recombinant SPP1 protein (Eton Bioscience) at 50 or 250 ng/mL.
Cell Division Phase Assay of hNSCs. To obtain the character of cell division and cell phase, we extinguished hNSC flow cytometry analysis using Pyronin Y and Hoechst 33258 staining.
Paolo Madeddu in the Bristol Heart Institute at the University of Bristol has used a conditionally immortalized clonal human neural stem cell (hNSC) line to treat animal models with limb ischemia and superimposed diabetes.
Although it is a relatively new material, its surface properties were shown to promote growth and proliferation of hMSC on a range of graphene-coated substrates  and to enhance the differentiation of human neural stem cells (hNSC) .
Both the Senate Armed Services Committee (SASC) and the House National Security Committee (HNSC) recommended an increase of $14 million to an Air Force $25.6 million R&D request to incorporate the needed improvements.
If the issue is primarily philosophical (rather than monetary), the House National Security Committee (HNSC) and the Senate Armed Services Committee (SASC) will usually take the initiative in stating directions to the DOD in their Defense Authorization Bill.
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