We examined whether non-cytotoxic concentrations (5-40 [mu]M) of sauchinone affected HO-1 protein expression, by treating HUVECs with this compound for 24 h.
Several studies have reported that nuclear translocation of activated Nrf-2 is an important upstream contributor to the mechanism of HO-1 expression in a wide variety of cells including endothelial cells.
Effects of sauchinone-induced HO-1 up-regulation on high glucose-induced cell adhesion molecules expression
We evaluated the influence of polymorphisms in the HO-1 gene on the risk of restenosis after percutaneous coronary stmt implantation in a cross-sectional study.
Multivariate logistic regression analysis was applied to assess the association of the HO-1 genotype, smoking, hyperlipidemia, DM, hypertension, age, and sex with the restenosis rate, to adjust for potentially confounding variables, and to test for potential interactions.
As shown in Table 1, the HO-1 +99G/C allele was present in 21 of the 199 (11%) patients investigated: 7 of the 97 (7%) who did not experience restenosis and 14 of the 102 (14%) who did (P = 0.
Rabbit polyclonal antibody CYP 2E1 and HO-1 was provided by Santa Cruz (California.
HO-1 activity was measured by determining the conversion rate of heme into bilirubin (Woo et al.
Western blot analysis for CYP2E 1 and HO-1 expression
Furthermore, a group of mice with boosted HO-1 activity and another group that directly inhaled carbon monoxide had substantially fewer inflammation-causing immune T cells in their brains than did mice getting a placebo.
The study also showed that boosting HO-1 activity reduces the accumulation of inflammatory T cells on myelin sheaths.