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Related to HOBT: HBTU, EDCI
HOBTHeart of the Beast Theatre (Minneapolis, MN)
HOBTHeap or B-Tree
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Abbreviations DIC: N,N'-diisopropylcarbodiimide DIEA: N,N-diisopropylethylamine DMF: N,N-dimethylformamide EDC: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide HOBt: 1-Hydroxybenzotriazole NHS: N-hydroxy succinimide PyBOP: Benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate TFA: Trifluoroacetic acid THF: Tetrahydrofuran TIPS: Triisopropylsilane TMSCl: Trimethylsilyl chloride.
"In many cases we'll have the actual demonstration going on in that aisle with the products, so the dietitian can show people the benefits of a particular food right there where that food is sold," Hobt says.
Another big movement at Hy-Vee is to have chefs working in the stores; Hobt estimates that the chain now has chefs in about 50 of its stores.
"Our service meat case is 36 feet and it kind of looks like a sea of meat, because some of our customers know ground beef and they know boneless, skinless chicken breasts, but all the other stuff can be intimidating because they really don't know what to do with it," Hobt says.
"Because sometimes they see raw fish over there and they may think it really looks great, but they have no idea what to do with it," Hobt says.
Many Hy-Vee stores supplement those offerings for much of the year with slabs of ribs grilled outside, which Hobt says is an example of the way stores are free to respond to customer demand.
Briefly, loading of the Fmoc-Gly-OH to Rink amide resin and all acylation reactions were carried out for 1 h using a 3-fold excess of Fmoc-amino acids activated with DIC (3.5 mmol) in the presence of HOBt (3.5 mmol).
HOBt (1.1 mmol) and DIC (1.1 mmol) were added to the solution of Boc-4-hydroxyproline (0.9 mmol) in dry THF at 0 oC, following the addition of Et3N (2.2 mmol) the mixture was stirred for 15 min.
The N termini were acylated by using acetic anhydride (10 mol equivalents) with HOBt (10 mol equivalents), HBTU (5 mol equivalents), and DIEA (10 mol equivalents) in DMF for 2 h.
(1) Nonstandard abbreviations: EM, erythema migrans; ACE, affinity capillary electrophoresis; HOBt, 1-hydroxybenzotriazol; HBTU, 2-(1H-benzotriazol1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate; DlFA, N-ethyldiisopropylamine; TFA, trifluoroacetic acid; DMF, NN-dimethlyformamide; BCIP/ NBT, 5-bromo-4-chloro-3-indolyl phosphate p-toluidine and nitroblue tatrazolium chloride; pNPP, p-nitrophenyl phosphate; dd, double distilled; PBST, PBS with Tween 20; MALDl-MS, matrix-assisted laser desorption ionization mass spectrometry; TBS, Tris-buffered saline; I[C.sub.50], concentration of soluble peptides at 50% inhibition; and AP, alkaline phosphatase.
Recently HOBT's artistic director, Sandy Spieler, began once again to fixate on water, this time in global terms--availability, quality, troubling claims of ownership.
And later this summer during Decorate the Well in Gratitude, the third and final episode, audiences can drink to the completion of HOBT's own home improvement project.