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Making use of our experience in the purification of TPST, we purified HPAT from solubilized Arabidopsis membrane fractions by affinity chromatography and identified 42 kD Golgi-localized type II transmembrane proteins that contained a single transmembrane domain near the N-terminus.
Our identification of Arabidopsis HPAT family arabinosyltransferases shed light on the function of their orthologs in leguminous plants such as Pisum sativum NOD3 and Medicago truncatula RDN1, whose loss-of-function mutants show hypernodulation phenotypes.
Similarly, tomato FIN, whose loss-of-function mutants show fasciated flowers associated with enlarged shoot apical meristems, has also been proved to be an HPAT family protein.
Because Hyp O-arabinosylation is also required for cell wall structural proteins, loss-of-function mutations in HPAT genes in Arabidopsis cause pleiotropic phenotypes that include enhanced hypocotyl elongation, defects in cell wall thickening, early flowering, early senescence, and defective growth of pollen tubes, causing a transmission defect through the male gametophyte.
Designed to perform safety checks in accordance with the IEE Code of Practice (4th Edition) the lightweight HPAT Series of rechargeable handheld testers are designed for ease of use, with one button automatic testing and clear connection details shown on the tester.
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