The use of higher performance capillary electrophoresis (HPCE
) has also been reported  as another technique which identifies, separate and quantifies intact bacteria.
We measured the MDA concentrations in plasma of patients undergoing coronary artery bypass grafting (CABG) by the TBA assay and by use of a commercially available lipid peroxidation kit (LPO-586, Bioxytech) and by HPCE
. MDA was undetectable by HPCE
Strengthened by a wave of new instruments, software, assay kits, and accessories, HPCE
is moving quickly into a wide variety of industries.
Analyses were carried out using a Hewlett Packard HPCE
system comprising a model HP3DCE apparatus interfaced to a HP Kayak XA workstation and a HP Laser Jet 4000 printer, and equipped with a HP fused silica capillary (# G 1600-61232; total length 56cm; effective length 50cm; i.d.
Picometrics ZETALIF high sensitivity laser induced fluorescence (LIF) detectors are the only LIF detectors that can be used for both HPLC and HPCE
. Detection limits can be as low as attomole or zeptomole levels, depending on the fluorescent label.
High-performance capillary electrophoresis (HPCE
) with cross-linked polyacrylamide gel offers the best performance in terms of resolution, and recently two groups [11,12] proposed such a technique for apo E genotyping.
Thanks to its speed, efficiency, and quantitative capabilities, high-performance capillary electrophoresis (HPCE
) could become the most effective quality control technique for biotechnology in the near future.
Water for HPCE
(part 5062-8578), 0.1 M sodium hydroxide for HPCE
(part 5062-8575), and 50 mM borate buffer (pH 9.3) for HPCE
(part 5062-8573) were from Hewlett Packard (HP; Palo Alto, CA, USA).
Now, however, thanks to lively scientific conferences, such as HPCE
'92 held recently in Amsterdam, Netherlands, a wide variety of additional uses for CE are becoming known.
HPLC's modern-day competitors are supercritical fluid chromatography (SFC), high-performance capillary electrophoresis (HPCE
), and possibly field-flow fractionation (FFF).