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Specimens were tested for enterovirus and HPeV RNA by reverse transcription PCR (RT-PCR) and primers specific for the highly conserved 5' untranslated region (1) (details for HPeV primers and probes are available on request).
pneumoniae], seven viruses [CMV, EV, HHV-6, HPeV, HSV-1/2, and VZV] and two fungi [Cryptococcus neoformans and C.
During 2009-2013, 2,724 specimens representing 2,532 patients tested for EV and HPeV were reported to NESS; the number of specimens submitted each year ranged from 392 in 2011 to 870 in 2012.
Willian Allan Nix from the Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, USA for providing the HPeV positive control.
For HPeV detection, we used a real-time reverse transcription PCR (RT-PCR) selective for the 5' non-translated region as described (13,14).
Enterovirus or HPeV serotypes were specified for 1,171 (72%) NESS reports.
During the study period, 46 patients had a diagnosis of severe diseases related to HPeV, determined by PCR analysis of serum or CSF samples.
The first viruses classified as HPeV (HPeV1 and HPeV2) were previously called echovirus 22 and echovirus 23.
On November 25, 2013, PCR detection of HPeV RNA confirmed HPeV infection in 2 of the children.
Human parechovirus (HPeV) is a newly classified genus ("Parechovirus") that was previously classified as enterovirus within the family Picornaviridae.
Whereas HPeV3 has been reported to be associated with sepsis-like syndrome, meningitis, encephalitis, and hepatitis in neonates and young infants (2), most HPeV infections are asymptomatic or associated with mild respiratory and/or gastrointestinal symptoms (4).
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