HPGFCHigh Performance Gel Filtration Chromatography
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The molecular weight (Mw) of MFPs was determined by high-performance gel-filtration chromatography (HPGFC), according to the method of Li et al.
The homogeneity and molecular weight (Mw) distributions of MFP-S and MFP-L were analysed by HPGFC. The average Mw of each elution peak was calculated according to the calibration curve derived from molecular weight standards.
Caption: FIGURE 3: HPGFC profiles of MFP-S (a) and MFP-L (b) monitored with a refractive index detector.