HPLC-DADHigh-Performance Liquid Chromatography with Diode-Array Detection
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PAH analysis was performed by HPLC-DAD (Waters, Milford, MA, USA) and followed our previous study [9].
According to the HPLC-DAD analysis, procedure I, during which the seedlings were cultured in MS medium for 10 days followed by culture in MS medium containing the desired elicitor for 20 days, led to 1.28 times higher rutin content in the H.
The foliar phenol profile of each species was made up of all compounds present in the respective HPLC-DAD chromatogram, treating each compound as a single chemical character.
HPLC-DAD separation of phenolic acids was accomplished according to previous methods with little alterations [9].
The authors identified anthocyanins using different methods: HPLC-DAD-ESI/MS and NMR, HPLC, HPLC-DAD [7].
HPLC-DAD analysis revealed that 5-CGA was the major phenolic acid ([greater than or equal to] 55.20%) in both skin and flesh of Moradita, Santa Maria, and Summerside cultivars (Table 1).
Leiva, Nonsteroidal Anti-Inflammatory Drug Determination in Water Samples by HPLC-DAD under Isocratic Conditions, J.
verrucosa confirmed that the genus is rich in bioactive flavonoids, due to the positive tests for flavonoids obtained by the reaction with Al[Cl.sub.3] and the analysis of the chromatographic profile obtained by HPLC-DAD. The detected flavonoids were responsible for the greater antioxidant activity of the leaves of the species and the steroids and saponins were responsible for the greater cytotoxic potential of the root.
Important flavonoids were identified by HPLC-DAD analysis and this study indicated that this plant extract has potent antioxidant and antimicrobial activities, as well as sunscreen action, important data for the future development of a new photoprotective formulation.
Ma, "Simultaneous quantification of eleven chemical components in traditional herbal medicinal formula socheongryongtang by HPLC-DAD and LC-MS," Journal of Liquid Chromatography and Related Technologies, vol.
Peaks for these compounds in HPLC-DAD spectrum were comprehensively determined by comparing their retention times and by coinjection of each sample with standards.