The sequences of PCR primers were as follows: [beta]-actin forward 5'-GTCATTCCAAATATGAGATGC GT-3' and [beta]-actin reverse 5'-GCTATCACCTCCCCTGTG TG-3' and HPSE forward 5'-CCTGAAGGCTGGTGG AG AAG-3' and HPSE reverse 5'-GGTAGCAGTCCGTCCA TTCA-3'.
Stably transfected HTR8/SVneo cells were constructed using an overexpression or a knockdown of the HPSE lentiviral vector.
The Effect of HPSE on Trophoblast Cell Tube Formation.
The Effect of HPSE on Trophoblast Cell Proliferation and Apoptosis.
Knockdown of HPSE Inhibited Trophoblast Cell Invasion by Activation of the p38 MAPK Pathway.
In contrast, no significant difference in survival was found according to age, HPSE gene expression, or STK11 or PTENgene status.
We also studied HPSE expression, which was found to be comparable in lung tumors and in nonaffected lungs.
 Human genes: F3, coagulation factor III (thromboplastin, tissue factor); KRAS, v-KI-ras 2 Kirsten rat sarcoma viral oncogene homolog; TP53, tumor protein p53; PTEN, phosphatase and tensin homolog; STK11, serine/threonine kinase 11; HPSE, heparanase; EGR1, early growth response 1; EGFR, epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian).
HPSE is synthesized as a proenzyme of 61.2 kDa, which is cleaved by cathepsin L to generate the active form consisting of 8 and 50 kDa subunits that associate noncovalently .
The results showed that low levels of HPSE activity could be detected in tears of healthy individuals and that in keratoconic individuals the level progressively increased as the Rabinowitz grade of the keratoconus increased (Figure 3).
HPSE is the sole human endoglycosidase that cleaves HS, although this molecule also displays various nonenzymatic activities [16, 17].
Whereas HS is produced by virtually all cells in the body, HPSE expression is kept tightly regulated at the transcriptional and posttranslational levels, since any uncontrolled cleavage of HS could result in significant tissue damage [14, 16].