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calcium infusion, partial healing in skeletal bone rickets findings were observed (Figure 1b); however, when the calcium infusion was stopped, secondary hPTH, increased ALP levels, and hypophosphatemia developed again and rachitic bone features became more apparent in the radiograms.
In our novel ON model, we established a new bone regenerative strategy that involves the synergistic effects from the concomitant use of local BMA transplantation and systemic hPTH administration.
 Nonstandard abbreviations: PTH, parathyroid hormone; hPTH, human PTH; PTHR-1, PTH receptor type 1; LC-MS/MS, liquid chromatography-tandem mass spectrometry; SPE, solid-phase extraction; NIBSC, National Institute for Biological Standards and Controls; [sup.15]NrPTH, [sup.15]N-labeled recombinant PTH; PTHrP, PTH-related protein; SRM, selected reaction monitoring; LOD, limit of detection; LOQ, limit of quantitation; ESRD, end-stage renal disease.
Half of the women were randomized to inject themselves daily with 400 IU of hPTH 1-34 and the other half injected placebo.
hPTH (1-34) has been produced in different forms including synthetic and recombinant.
Serum intact parathyroid hormone (1-84, hPTH) was measured in duplicate with a commercial kit (N-tact PTH IRMA Kit, Incstar Inc., Stillwater, MN, USA) using a two-site immunoradiometric assay as described by Lindall et al.
Additionally, the difference in the amount of hPTH (1-84) calibrators present in the assays is probably one of the major factors that contribute to the differences in the percentage recoveries and slopes.
 Nonstandard abbreviations: PTH, parathyroid hormone; I-PTH, intact parathyroid hormone; hPTH, human parathyroid hormone; T-PTH, total parathyroid hormone; CA-PTH, cyclase-activating parathyroid hormone; and E-PTH, Elecsys parathyroid hormone.
Thus, peptides lacking the N-terminal serine of hPTH fail to register in these new assays, whereas the detection antibodies used in the first-generation IRMAs bind N-truncated peptides, such as PTH(7-84), just as well as those with an intact NH, terminus (14,15).
 Nonstandard abbreviations: PTH, parathyroid hormone; hPTH, human parathyroid hormone; C-PTH, carboxyl-terminal parathyroid hormone; I-PTH, intact parathyroid hormone; CA, cyclase-activating; and T-PTH, total parathyroid hormone.
Elecsys parathyroid hormone (PTH) not detecting the large PTH fragment hPTH (7-84)?
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- HPT-1 cadherin