Anti-hemolytic (anti-venom) activity by human red blood corpuscles (HRBC) membrane stabilization method: Venom induced hemolysis and its neutralization by medicinal plants of Pakistan was carried out by following the method of Vijayabharathi et al (2005).
Venom (100 g/mL 1mL) Phosphate buffer (0.15 M pH 7.4 1mL) and HRBC (1% v/v 1mL) were mixed and incubated at 37 C for 30 minutes.
1) was found to destabilize HRBC membrane resulted in hemolysis as seen with Naja naja (patterned form widespread in Sind province as shown in fig.
Standard anti-sera and Enicostemma hyssopifolium (Willd.) Verdoorn extract at lower concentration (20 g/mL) was found more protective to HRBC membrane as compared to their higher concentrations (40-320 g/mL).
Sixty seven percent plants were unable to prove scientifically (p0.05) their folklore potential as anti- hemolytic however; seven percent were active to stabilize HRBC having p0.1.
Absorbance of hemoglobin release due to hemolysis of HRBC by various hemolytic agents.
Effect of application incubated mixture of different anti-dote (plant extracts / antiserum) and Naja naja karachiensis venom on isolated HRBC (human red blood corpuscles) membrane stabilization.
As a result HRBC membranes destroyed and change the environment of target protein(s) therefore resulted in agonistic antagonistic effects and additionally posed interference in binding with physiologic ligands.
As part of Mamata's ` 250- crore makeover plan for the 236- year- old Writers' Buildings that currently houses several state departments, altogether 28 departments, including the chief minister's office ( CMO), will be shifted to the HRBC building in Kolkata's twin district Howrah.
Mamata is said to have been struck by the sight of the HRBC building while returning from a political rally in June.