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Characterization of HSLNs. In both HSLNs, the potency of active constituents was in the range of 90 to 110 % of the expected theoretical potency.
Statistical analysis using unpaired t-test reveals that there was a significant difference (p<0.05) between the particle sizes obtained for beeswax and GMS HSLNs (Figure 2(e)).
Therefore, it is expected that beeswax HSLNs will demonstrate lower degree of crystallinity upon cooling and storage as compared to GMS HSLNs.
Study of Antidepressant Activity in Swiss Albino Mice Using Beeswax HSLNs
Compared to control group, HSLNs (200 mg/kg) treated group showed significant difference in the immobility time at significance value of P < 0.05 (Figure 3(a)).
In the swimming time test (Figure 3(c)), the test group treated with 100 mg/kg HSLNs also demonstrated a significantly ([alpha] = 0.05) higher swim time as compared to the control group.
For FST immobility time study, data analysis using one-way ANOVA followed by Bonferroni's multiple comparison post hoc test showed that there was no statistically significant difference between crude extract mean and means obtained for 10 and 50 mg/kg HSLNs treated groups (Figure 3(a)).
In other words, the immobility time for 10 and 50 mg/kg HSLNs treated groups was not significantly different than the crude extract treated group (200 mg/kg).
In this test, significant reduction in immobility times (one-way ANOVA, followed by Dunnett's multiple comparison test) was seen at 100 mg/kg and 200 mg/kg HSLNs treated groups when compared with the control group (Figure 3(d)).
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