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Tissue samples were homogenized with a motor-driven homogenizer in acetate buffer (8 mM, pH 5.4) containing HTAB. For evaluation of MPO activity, the supernatant was incubated with TMB (18.4 mM) at 37[degrees]C for 3 min.
The two men were washed away by Wadi htab waters, in Laarich locality, while they were on board their car stuck in the flooded Wadi after heavy showers in Kasserine, the night of October 5, 2016.
The colon tissue was weighed and homogenized in 1mL of 50 mM potassium phosphate solution (pH: 6) with 0.5% HTAB and 5 mM EDTA in an ice bath using polytron homogenizer.
For MPO estimation, granulation tissue (5% w/v) was homogenized in 0.5% HTAB with 50 mM potassium phosphate buffer (pH 6).
And the conductivity of polymer solution increased from 167 [micro]s/cm to 1104 [micro]s/cm, as HTAB surfactant concentration increased from 3.5 x [10.sup.-3] mol/L to 3.5 x [10.sup.-2] mol/L.
The assay of MPO activity was performed via the HTAB method.
The OMMT were prepared according to a standard ion exchange procedure by using HTAB as cationic surfactant.
The following command string might recur throughout a case simulation program: VTAB 12: HTAB 5: INVERSE: PRINT "THAT'S CORRECT"; N$: NORMAL: GOTO.
The pellets were then collected and were homogenized in 10 volumes of ice cold 50 mM potassium phosphate buffer (pH 6) containing 0.5% hexadecyl-trimethyl ammonium bromide (HTAB) and 10 mM EDTA.
In brief, frozen lung specimens were weighed and homogenized in hexadecyltrimethylammonium bromide (HTAB) buffer (0.5% HTAB in 50 mM phosphate buffer, pH 6.0).
Pellets were resuspended in a cooled 0.05 M potassium phosphate buffer (pH 6) containing 0.5% hexa-1,6bis- decyltrimethylammonium bromide (HTAB) and homogenized.
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