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The HTFs market is driven by various factors such as high demand from the chemical industry, increasing the need for energy conservation, and high performance of HTFs.
Synthetic fluids are the fastest-growing segment of the HTFs market, by type.
The increasing developments related to chemical, automotive, oil & gas, CSP, bio-diesel production, and HVAC & refrigeration industries in China, India, Japan, and South Korea provide huge opportunities for the HTFs market.
New product launch and expansion were the key strategies adopted by players in the HTFs market to increase their product portfolio and global presence to cater to the growing demand.
- High Potential for the Development of Bio-Based HTFs
HTFs were obtained as an expansion culture of the Tenon capsule explants of 1 x 1 [cm.sup.3] and were propagated in Dulbecco's modified Eagle medium (DMEM, Invitrogen, Carlsbad, CA, USA) supplemented with 15% heat-inactivated fetal bovine serum (FBS, Hyclone, Logan, UT, USA), 100U/mL penicillin, and 100 [micro]g/mL streptomycin (Sigma-Aldrich, Saint-Louis, Missouri, USA) in 5% C[O.sub.2] humidified atmosphere at 37[degrees]C.
The HTFs were transfected with CTGF-siRNA (50 nM) or control siRNA (50 nM) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol.
Quantitative real-time PCR was used to determine the level of CTGF mRNA of HTFs after various treatments.
HTFs were seeded in coverslips before transfection of siRNA.
The effect of CTGF-siRNA on HTFs viability after TGF-[[beta].sub.2] treatment was determined by cell counting kit-8 (CCK-8, Biomics, Nantong, China) assay.
At one extreme, they cover HTFs operating in sectors such as biotechnology: at the other, priority status is accorded to firms that utilize new technology in more conventional sectors, for example food processing.
Nanofluids have been shown to be superior, as a HTF, to conventional known fluids available in the market.
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