HTPOHansen Thorp Pellinen Olson, Inc (Eden Prairie, MN)
HTPOHad To Play On (online puzzles and riddles community)
HTPOHanford Transition Program Office
HTPOHuman Technology Performance Optimization (Imago Metrics LLC)
References in periodicals archive ?
The DNA was transferred onto a positively charged nylon membrane (Roche) and hybridized with the DIG-labeled hTPO cDNA fragments.
The first PCR primers for the hTPO cDNA were as follows: 5'-ggagctgactgaattgctcctcgt-3' and 5'-cctgacgcagagggtggaccctcc-3'.
We constructed two knock-in vectors to direct expression of the hTPO gene under control of the endogenous beta-casein promoter at the bovine beta-casein locus (Figure 1A and B).
clones were further confirmed by Southern blot analysis; a specific 9.9-kb signal band was detected after hybridization using a probe from the hTPO cDNA (Figure 2E).
Transgenes of cloned embryos were analyzed at various developmental stages by PCR amplification for hTPO. Twenty-three (88%) of 26 cloned embryos derived from KI-81 cells and 43 (83%) of 52 from KI-89 cells were positive for the transgene (Figure 3A and Table 2).
In this report, we successfully achieved, for the first time, knock-in of the hTPO gene on the beta-casein locus of bovine fibroblasts.