Subsequently, the membrane was incubated with primary antibodies (HtrA
: ab38610, Notch-1: ab52627, diluted 1:1000 in PBS; Abcam, USA) followed by the appropriate peroxidase-coupled secondary antibody (#58802, diluted 1:2000 in PBS; Cell Signaling Technology, USA).
The efficiency of qPCR assay for detection of 47-kDa HtrA
gene and 17-kDa gene was 97% and 101.3%, respectively.
serine proteases as potential therapeutic targets in cancer," Current Cancer Drug Targets, vol.
are keen to work together to demonstrate that young people can make a positive contribution to their area.
The final supermatrix consisted of 2,308 bp (731 bp for gtlA, 429 bp for ompA, 744 bp for ompB, and 404 bp for htrA
genes), with 670 variable sites, 494 singletons, and 176 parsimony informative sites.
All the sequences generated for the ompA and htrA
genes showed 100% identity to sequences from the Rickettsia parkeri strain Portsmouth (GenBank accession no.
6-OHDA: 6-hydroxydopamine; Bak: Bcl2 antagonist/killer; Bax: Bcl2 associated X; Bcl2: B cell leukemia/lymphoma 2; Cdk5: cyclin-dependent kinase 5; Cyt C: Cytochrome C; IAPs: inhibitors of apoptosis proteins; [MPP.sup.+]: 1-methyl-4-phenylpyridinium; Omi/HtrA2: HtrA
serine peptidase 2; ROS: reactive oxygen species.
HTS competitive binding assays for human, bovine, and mouse estrogen receptor (hER, bER, mERa, respectively), rodent and human androgen receptor (rAR and hAR, respectively), human thyroid hormone receptor-alpha (hTRa
), and a human aromatase (hCYP19A1) enzyme inhibition assay (as part of a larger collection of 239 targets) were developed and run by Caliper Discovery Alliances and Services (Hanover, MD).
proteases: regulated proteolysis in protein quality control," Nature Reviews Molecular Cell Biology, vol.
(i.e., a 185-bp  and a 397-bp [4,5] fragment of the 16S rRNA gene) were amplified; the sequence of the 397-bp fragment was 100% similar to the htrA
sequence of the B.
henselae DNA was detected by using PCR with specific primers for the 16S-23S rRNA intergenic spacer (9) and the htrA
gene (10), and the 16S rRNA genotype was confirmed by partial sequencing of the 16S rRNA gene (11).