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To do this, the HTrF values are first presented to capture the uncertainty of FLEs.
This section recalls briefly the definition of fuzzy linguistic approach and the basic concepts of HTrF values.
Xie, "HTRF: a technology tailored for drug discovery--a review of theoretical aspects and recent applications," Current Chemical Genomics, vol.
Abbreviations NAFLD: Nonalcoholic fatty liver disease ETS: Extract of Tamarindus indica seed coat NASH: Nonalcoholic steatohepatitis ROS: Reactive oxygen species ND: Normal diet HFD: High fat diet NISCAIR: National Institute of Science Communication and Information Resources FFA: Free fatty acids AST: Aspartate aminotransferase ALT: Alanine amino transferase MDA: Malondialdehyde VLDL: Very low density lipoproteins H&E: Hematoxylin and eosin LPO: Lipid peroxidation HTRF: Homogeneous time resolved fluorescence HOMA: Homeostatic model assessment SREBP-1c: Sterol regulatory element binding protein-1c IRS-1: Insulin receptor substrate-1 JAK: Janus activating kinase SOCS-3: Suppressor of cytokine signaling-3 CREBP: Carbohydrate response element binding protein.
This tool is another example of why Cisbio Bioassays and HTRF have become synonymous with R&D innovation in the GPCR arena, as our portfolio enables researchers to investigate GPCRs from an increasing number of angles."
The company pioneered the field of homogenous fluorescence methodologies via its proprietary technology, HTRF, a highly sensitive, robust technology for the detection of molecular interactions and widely used by the pharmaceutical industry for the high throughput screening stage of drug development.
This was performed in cell free system using HTRF Sirt-1 deacetylaion kit following manufacturer's instructions.
The anti-cortisol cryptate and the d2 labelled cortisol will bind to each other and results in a high HTRF signal.
For example, the Packard HTRF lck assay has a lower detection limit of 2 pmol/L (11).
[PGE.sub.2] levels in macrophage supernatants were quantified as per the method described by the kit manufacturer [Homogenous Time Resolved Fluorescence (HTRF) kit, CisBio, France].
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