15 samples of hUCB
and 15 samples of hUCWJ were taken for the purpose of the study after due approval of the Institutional Ethics Board (SMIMS/IEC/C/2016-069) from the placental ends after Caesarean section.
When the team knocked out Ythdf2 function in a mouse model or knocked down Ythdf2 function in hUCB
cells, they observed increased expression of these transcription factors and expansion of hematopoietic stem cells, which are the major type of adult stem cells in hUCB
Conclusion: Based on our results, the in vitro expansion of HUCB
resulted in altered expression levels of miRNAs.
According to Eduardo Cruz, CEO of Cell PRAXIS BioRio, human umbilical cord blood cells (hUCBs
) are limited to collection at the time of birth, but menstrual blood-derived stem cells (MenSCs) could be collected once a month for 40 years from women during their reproductive stage.
Previously, we formulated a freezing medium containing trehalose, ectoine, and catalase for effective cryopreservation, and encouraging results were obtained with human umbilical cord blood (hUCB
) mononuclear cells [30,31].
The study shows that HUCB
neuroprotection is dependent on the activation of the Akt signaling pathway that increases the transcription of the Peroxiredoxin-5 (Prdx5) gene.
cells have received attention as an alternative source of stem cells that have been studied and shown to be effective with wide therapeutic potentials, how the cells might be used to repair the diseased, as well as normally aging brain, has become an important question.
Under sterile conditions, HUCB
was collected from fresh placentas with the umbilical cord still attached.
Laboratory culture studies examining the activity of human umbilical cord blood (HUCB
) cells on experimental models of central nervous system aging, injury and disease, have shown that HUCB
cells provide a "trophic effect" (nutritional effect) that enhances survival and maturation of hippocampal neurons harvested from both young and old laboratory animals.
The study found that HUCB
cells were not only able to protect hippocampal neurons taken from the brains of young adult and aged rats, but also promoted the growth of dendrites - the branching neurons acting as signaling nerve communication channels - as well as induced the proliferation hippocampal neurons.
was collected from umbilical veins after neonatal delivery after obtaining informed maternal consent.
The frequency of MSCs in hUCB
is a point of controversy among researchers [56, 57, 107].