Recently, it has been observed that HUCB contains various stem progenitor cells with the ability to differentiate into both hematopoietic and non-hematopoietic cells.
Our study is the first study on miRNA expression profiles in HUCB cells after in vitro culture compared with that in fresh isolated [CD133.
hematopoietic cells from HUCB (Sample 1) were spherical, and their purity analyzed by flow cytometry was 98% (figure 1).
This finding suggests that HUCB cell therapy could be a promising treatment for stroke and other forms of brain injury when gene expression is modified to promote neural protection," Willing said.
Prdx5 may be an important component of the antioxidant effect of HUCB cells and, therefore, a candidate for therapeutic targeting of oxidative stress in stroke.
The researchers concluded that HUCB cells "rescued" neural cells and prevented neuronal loss in rats modeled with stroke by increasing the transcription of genes related to survival and repair by inhibiting inflammation.
They concluded that HUCB cells benefit aging adult hippocampal neurons by 'increasing their survival, growth, differentiation, maturation and arborization' (branching).
The mechanisms by which HUCB cells extend the life of hippocampal cells may include enhancing the proliferative capacity of the cells or protecting existing and newly generated neurons from damage and death," concluded Willing.
The study found that HUCB cells were not only able to protect hippocampal neurons taken from the brains of young adult and aged rats, but also promoted the growth of dendrites--the branching neurons acting as signaling nerve communication channels--as well as induced the proliferation hippocampal neurons.
The researchers also reported that the difference between HUCB cell-treated cultures and non-treated cultures was "more dramatic in the older adult rat brain cultures" than in those younger rats.
A decade of studies using animal models of stroke has found that in many cases hUCBs failed to enter the brain following transplantation, yet behavioral improvements were often observed, Borlongan said.
Similarly, studies using animal models of Alzheimer's disease have found that hUCBs also play an anti-inflammatory role.