However, given the complexity of vasculature, further animal studies are required to confirm the findings obtained using HUVEC
cell model and to elucidate the responses of lymphocytes and leukocytes to RA-supplemented HDF in vascular tissues.
were cultured in a 5% CO[sub]2, 37[degrees]C water-saturated atmosphere.
These results suggest that A2 can decrease gastric cancer cell proliferation without cytotoxic effects on both noncancerous endothelial cell HUVEC
and epithelial cell HEK-293 cells.
coli 15, serogroup O153, (3) was selected among other SEPEC strains because this strain induced more intense cytotoxic effects on HUVEC
angiogenic tube formation assay on extracellular matrix in the presence of 1% venous umbilical cord plasma from PI pregnancies.
(Clonetics/Lonza, Basel, Switzerland) were maintained in complete medium (EGM-2; Lonza) composed of endothelial cell basal medium (EBM-2, Lonza) supplemented with endothelial cell Bullet Kit which contains 2% foetal calf serum (FCS), human epidermal growth factor-(EGF-) 2, human fibroblast growth factor-2 (FGF-B), human vascular endothelial cell growth factor (VEGF), R3-insulin-like growth factor-1 (R3-IGF-1), ascorbic acid, hydrocortisone, heparin, gentamicin, and amphotericin B.
The spiked HUVEC
count was categorized into 3 groups: 0, 20, and 200 cells, with each group containing 3 replicates of tests.
In one study (60) the proa-ngiogenic miR-130a is expressed at low levels in quiescent HUVEC
and is up-regulated in response to foetal bovine serum.
2007) found that RA decreases in vitro VEGF secretion by thyroid cancer cell lines and inhibits HUVEC
proliferation, which was dose-dependent.
Gold nanoparticles inhibit VEGF165-induced proliferation of HUVEC
las de estroma y las HUVEC
, pero no las celulas comprometidas de linaje mieloide y linfoide, tambien expresan tres factores transcripcionales Gli-1, Gli-2 y Gli3, que actuan corriente abajo de la cascada de senalizacion Hh (48), sugiriendo que posiblemente la senalizacion Hh no es esencial para la maduracion hematopoyetica.
It was observed that the initial cell adhesion was comparable onto bare and coated stents, whereas the rate of proliferation of HUVEC
was minimal when grown on drug coated stent as compared to that on bare metal stent (p<0.