The spiked HUVEC
count was categorized into 3 groups: 0, 20, and 200 cells, with each group containing 3 replicates of tests.
were grown on 1% gelatin coated tissue culture plates in MCDB 131(Gibco BRL), containing 20% Calf serum, antibiotics and 200[micro]g/ml Endothelial cell growth factor (ECGF), in a humidified incubator under 5% C[O.
from spinner cultures displaye d strong angiogenic response that was increased by SNAP.
Abbreviations EC epicatechin ECG epicatechin gallate ECC epigallocatechin EGCG epigallocatechin-3-gallate eNOS endothelial nitric oxide synthase HO-1 heme oxygenase-1 ICAM1 intercellular adhesion molecule-1 JNK c-Jun N-terminal kinase HUVEC
human umbilical vein endothelial cells 3-MA 3-Methyladenine MCP1 monocyte chemoattractant protein-1 NO nitric oxide PAI-1 plasminogen activator inhibitor-1 ROS reactive oxygen species TNF-[alpha] tumor necrosis factor alpha VCAM1 vascular cell adhesion molecule-1 VEGF vascular endothelial growth factor vWF von Willebrand factor
Because angiogenesis is essential for tumor progression and metastasis, we used HUVECs
to examine the influence of chronic B[a]P exposure on tumor angiogenesis.
The effects of Ankaferd I; Blood Stopper on transcription factors in HUVEC
and erythrocyte protein profile.
786-0 cells were purchased from ATCC, and HUVECs
were obtained from Cambrex.
We expect that elevated levels of NO produced from SNAP will modify the angiogenic response of HUVEC
, in our 3-dimensional model, to CM from breast cancer cells.
EphB4 inhibits HUVEC
cell growth through suppression of the Ras/MAPK pathway.
Cryptotanshinone, a lipophilic compound of Salvia miltiorrriza root, inhibits TNF-alpha-induced expression of adhesion molecules in HUVEC
and attenuates rat myocardial ischemia/reperfusion injury in vivo.
were cultured in EBM-2 complete medium, and switched to EBM-2 basal medium containing 0.
We also suggest that more information about the in vivo scenario can be gained with further insight into the use of human cord blood stromal cells or HUVEC
(human umblical vein endothelial cells) as feeder layers and with exploration of in vitro behavior of LTC-IC for CFC production.