To check whether inhibition of blood vessels development by CLT in vivo was due to direct inhibition on endothelial cells, we assessed the effects of CLT on tube formation of HUVECs
Transwell plates containing HUVECs
in the top chambers and conditioned medium from B[a]P-exposed BEL-7404 cells in the bottom chambers were incubated for 6 hr; bars = 150 [micro]m.
It was observed that the initial cell adhesion was comparable onto bare and coated stents, whereas the rate of proliferation of HUVEC
was minimal when grown on drug coated stent as compared to that on bare metal stent (p<0.
were cultured in EBM-2 complete medium, and switched to EBM-2 basal medium containing 0.
2 shows density plots of the mixture of HUVECs
multistachys Chloranthus multistachys DMEM Dulbecco's minimum essential medium DMSO dimethyl sulfoxide ECM extracellular matrix EGF epidermal growth factor EMT epithelial-mesenchymal transition FBS fetal bovine serum HUVECs
human umbilical vein endothelial cells IR infrared radiation ISTA istanbulin A MMP matrix metalloproteinase MS mass spectrometer MTT 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide NMR nuclear magnetic resonance OPN osteopontin Runx2 Runt-related transcription factor 2
were subsequently treated with dihydro-rhodamine 123 (Molecular Probes; Invitrogen) at a final concentration of 10 [micro]M for 30 min at 37[degrees]C in 5% carbon dioxide, rinsed with Earle's balanced salt solution, and immediately analyzed (excitation, 485 nm; emission, 530 nm).
Similar results were obtained for HUVECs
after treatment with Ln[P0.
In the proliferation assay, the researchers first incubated HUVECs
for 48 hours and exposed them to varying concentrations of exisulind and CP461, both with and without the growth stimulators VEGF and bFGF.
Treatment of HUVECs
with PA resulted in decreased phosphorylation of ribosomal S6 and 4EBP1 proteins, the two downstream effectors of mTOR signaling.
Stimulation of cultured HUVECs
with 50 mm 1 mL of GFBS significantly enhanced lipid peroxidation and decreased antioxidant enzyme activities and levels of phase II enzymes.
In vitro, incubation of HUVECs
with particulate matter collected from air sampling has been reported to increase E-selectin production (Alfaro-Moreno et al.