Comparative evaluation of maintenance of cell viability of an experimental transport media" coconut water" with Hank's balanced salt solution
and milk, for transportation of an avulsed tooth: An in vitro cell culture study.
Felippe, "Fibroblast viability after storage at 20 degrees C in milk, Hank's balanced salt solution and coconut water," Brazilian Dental Journal, vol.
Caption: Figure 3: Total cell number and cell viability of ADSCs after preservation for 16 h in Hank's balanced salt solution (HBSS) or University of Wisconsin (UW) solution.
The electrolyte used for simulating human body fluid conditions was Hank's balanced salt solution
, which was prepared using laboratory grade chemicals and double distilled water.
If they could choose a better storage medium, they would use Hanks' balanced salt solution, an option that is well documented in the literature.18 Comparing milk with Hank's Balanced Salt Solution
(HBSS), authors noted that HBSS demonstrated better results, even when the teeth were maintained for twelve hours.19
Each swab was collected in 1 mL viral transport medium (1x Hank's balanced salt solution
containing 5% fetal bovine serum, penicillin [100 U/mL], and streptomycin [100 [micro]g/mL], pH 7.4) in 5-mL cryovials.
( ) Normal saline ( ) Milk ( ) Hank's balanced salt solution
( ) water ( ) saliva or other: please specify
If a cytopathic effect (CPE) was not evident within 7 days, cell monolayers were washed with Hank's balanced salt solution
(HBSS) six times to eliminate viral particles from the primary extract or from infected cells.