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References in periodicals archive ?
Sen, "A comparative study of clinically suspected syphilis cases with serological test VDRL/RPR & Treponema pallidum hemagglutination assay (TPHA) in a tertiary care hospital (Silchar Medical College & Hospital)," Journal of Science, vol.
Confirmation testing that the laboratories recommended and/or performed reflexively after a positive initial screen (question 5) includes 34.7% (615 of 1774) that use the fluorescent treponemal antibody-absorption test, 23.1% (410 of 1774) that use an agglutination or hemagglutination assay, and 23.2% (412 of 1774) that use an RPR assay.
Keywords: Measles, Serodiagnosis, Indirect Hemagglutination Assay, Humoral Immune Response.
Hemagglutination assays were performed using human (ABO groups), sheep and rat blood in a U-bottomed 96-well microtiter plate.
VDRL (Venereal Disease Research Laboratory) and TPHA (Treponema pallidum hemagglutination assay) results were nega-tive.
The dead eggs were removed from the incubator, chilled and harvested and used for hemagglutination assay. At the end of the 4 days the remaining eggs were chilled overnight and harvested and also quantified by hemagglutination assay.
Serologic testing for Trypanosoma cruzi: comparison of radioimmunoprecipitation assay with commercially available indirect immunofluorescence assay, indirect hemagglutination assay, and enzyme-linked immunosorbent assay kits.
The American Red Cross (ARC) National Testing Laboratory (NTL) (identified as the "American Red Cross National Donor Testing Laboratory" in the article) has never performed indirect hemagglutination assay testing, a method not licensed by the Food and Drug Administration (FDA) for detection of antibodies to Trypanosoma cruzi.
Hemagglutination Assay. Twofold serial diluted 1 O[D.sub.600] equivalent EBY100/pYD1-HA were added to 12-well plates, followed by addition of 1% chicken red blood cells.
Due to the possibility of a false positive result caused by a viral infection, tuberculosis, or connective tissue diseases, confirmatory testing with fluorescent treponemal antibody absorption (FTA-ABS) or a Treponema pallidum hemagglutination assay (TPHA) is necessary.
The pH dependence of the lectin was determined by incubating 50 [micro]g of LCL with buffers in different pH:HC1: pH 0-1, glycine/HCI (pH 2-3), 0.05 sodium acetate/acetic acid (pH 4-5), potassium phosphate (pH 6-7), Tris-HCI (pH 8-9) and glycine-NaOH (pH 10-11), pH 11-12 NaHC[O.sub.3] and pH 13-14, NaOH, for 5 h at 25 [degrees]C and pH was adjusted to 7.2 just prior to hemagglutination assay.
The TPHA (Treponema Pallidum Hemagglutination Assay) also from the Biomerieux Laboratory is a confirmatory treponemic method that involves red blood cells treated against the presence of the treponema in the sample and a hemagglutination reaction on the slide; it is simple to perform, is read visually and its accuracy similar to that of the immunofluorescence technique except in primary syphilis (chancre present) in which its sensitivity and specificity do not surpass 89%.
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