HILICHydrophilic Interaction Chromatography (HPLC)
HILICHydrophilic Interaction Liquid Chromatography
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Moreover, targeted analysis improves reproducibility, and the application of UHPLC enabled analysis time to be decreased compared with traditional HILIC separations that require long column equilibration times (data not shown).
HILIC at present attracts much attention be-cause it solves many problems of previously difficult separations.
Columns (Waters XBridge Amide, 2.5 [micro]m, 2.1 x 150 mm and Agilent Poroshell 120 HILIC, 2.7 [micro]m, 2.1 x 150 mm), column temperature (20, 25, 30, 35, and 40[degrees]C), mobile phase (acetonitrile-0.1% formic acid, acetonitrile-0.2% formic acid, acetonitrile with 0.2% formic acid-0.2% formic acid, and acetonitrile-10 mM ammonium formate with 0.2% formic acid), and different kinds of gradient elution were investigated.
HILIC 2016 Lopes, [33] Untargeted [sup.1]H NMR Thiago I.B.
In the HILIC technique, 2 [micro]L of the sample solution was injected onto the column (Acquity UPLC BEH Amide 1.7 [micro]m, 2.1 x 100 mm, Waters Corporation, Milford, MA, USA) and maintained at 45[degrees]C.
All the samples were coded with numbers and a blind random analysis was conducted by a separate analyst on five different days on the developed HILIC method and by the method reported by Yuzuk et al.
RP-HPLC-QTOF-MS and HILIC-QTOF-MS: reversed-phased (RP) high liquid chromatographyand hydrophilic interaction chromatography(HILIC) coupled with quadrupole time-of-flight mass spectrometry.
Lv et al., "Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations," Analytica Chimica Acta, vol.
Four New Types Developed for SEC and HILIC Chromatography
Then a targeted analytical method for 106 polar metabolites was developed using a HILIC column which has a strong retention to polar compounds and triple quadrupole mass spectrometer.
The chromatography columns market on the basis of phase is sub segmented into reverse phase (RP) columns, ion exchange phase column, size exclusion columns, chiral separation columns, affinity columns, normal phase/hydrophilic interaction columns (HILIC), other chromatography columns.