Shortly after being injected, mice quickly developed large tumors, but when researchers used a biologic technique ("knockdown") to reduce hnRNP A2/B1 before injection, the mice developed only small tumors or no tumors at all.
These results suggest that hnRNP A2/B1 is a driving oncogene--a gene that causes normal cells to become cancerous--on its own and probably directly contributes to glioblastoma development," said Rotem Karni, Ph.
Karni said that "over expression and amplification of hnRNP A2/B1 correlate with poor prognosis of glioma patients, whereas deletion of the hnRNP A2/B1 gene correlates with better prognosis than average.
These biochemist researchers are continuously trying to identify the genes which are regulated by hnRNP A2/B1.
TDP-43'un, hucre-ici kompartmanlar, hucre turleri ve hastalik evresine gore degisiklik gosteren buyuk hnRNP
kompleksleri cercevesinde gorev yapmasi da olasidir.
hnRNP A1, an mRNA splicing regulator, exclusively stimulates maturation of miR-18a from the miR-17-92 cluster.
Recently, it was discovered that miR-328 has a second function, acting as a decoy by binding to hnRNP E2 and lifting its translational repression of an mRNA involved in myeloid cell differentiation .
miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts.
In addition, the research team discovered that the hnRNP
A2/B1 protein, in and by itself, can transform normal cells into cancerous ones, and inhibition of the protein prevents tumor development in mice.
The effects of eurycomanone treatment on the expression levels of known lung cancer markers, heterogeneous nuclear ribonu-cleoprotein A2 and its splicing variant Bl (hnRNP A2/B1), were examined by immunoblotting using antibodies specific for hnRNP A2/B1.
Results from our study further showed that eurycomanone also targets other pathways affecting cell replication notably those involving p53 tumor suppressor gene, the lung cancer cell markers hnRNP A2/B1, annexin I and prohibitin.
Molecular characterization of the hnRNP A2/B1 proteins: tissue-specific expression and novel isoforms.