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References in periodicals archive ?
Presumptive colonies were streaked onto horse blood agar and TSA plates and incubated at 35AdegC for 48 h.
Hemolytic activity was done on horse blood agar (BM,[C], Malaysia).
All strains showed [beta]-hemolysis on horse blood agar. But, two isolates of A.
in 2006 compared the efficacy of agars that used citrated sheep blood agar and outdated human blood agar with defibrinated horse blood agar and defibrinated sheep blood agar for the isolation and antibiotic susceptibility testing of reference and clinical strains of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus.
One hundred microlitres of this fluid was inoculated onto 5% horse blood agar, then incubated aerobically in 5% C[O.sub.2] at 35[degrees]C for 48 hours.
We seeded synovial fluid on blood culture, and small colonies developed on horse blood agar. We extracted DNA from the zone of hemolysis using EZ1 DNA tissue kit (QIAGEN, Valencia, CA, USA).
Placental swab samples were cultured on Columbia horse blood agar (CBA) and chocolated CBA (both incubated aerobically with 5% CO2 at 37[degrees]C for 24 hours), cysteine lactose electrolyte deficient agar (incubated in air at 37[degrees]C for 24 hours), and fastidious anaerobic agar with and without neomycin (incubated anaerobically at 37[degrees]C for 48 hours); all agar was from Thermo Fisher, Basingstoke, UK.
The isolated strain showed slight growth after 24 or 48 h of incubation on horse blood agar medium but abundant growth on the same medium containing 1% Tween 80 under aerobic conditions (5% C[O.sub.2]).
Sputum cultures were plated onto horse blood agar, cystine lactose electrolyte-deficient agar, Haemophilus agar, and colistin/ nalidixic acid blood agar incubated in a candle jar at 35[degrees]C for [less than or equal to] 3 days.
At 24 h, strong [beta]-hemolysis was produced by 55 (76%), 41 (57%), and 55 (76%) strains on human, sheep, and horse blood agar plates, respectively, while 6 (8%), 10 (14%) and 7 (10%) strains demonstrated weak [beta]-hemolysis (Table).
On sheep and horse blood agar, a thin line of annular hemolysis was observed 4-12 mm from the colony edge.