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Expression of hsp 27, hsp 60, hsc 70, and trip 70 mRNA and protein in UROtsa cells acutely exposed to NaAs[O.sub.2].
Expression of hsp 27, hsp 60, hsc 70, and hsp 70 mRNA and protein in UROtsa cells acutely exposed to heat shock.
Expression of hsp 27, hsp 60, hsc 70 and hsp 70 mRNA and protein in UROtsa cells exposed to NaAs[O.sub.2] for 16 days.
For hsp 27 there was no change in the expression of mRNA over the initial 13 days of the time course for all three concentrations of NaAs[O.sub.2] (Figure 7A).
Thus, UROtsa cells express basal levels of mRNA for the hsp 27, hsp 60, hsc 70, and hsp 70A genes regardless of growth media composition, but demonstrate reduced relative levels of mRNA expression for the hsp 70A gene when grown on serum-free media.
In agreement with the basal expression of hsp 27 mRNA, an analysis of hsp 27 protein expression by Western blotting demonstrated that the basal expression of the hsp 27 protein was approximately equal when the cells were propagated on either growth media (Figure 5).
A similar analysis of hsp 27, hsp 60, hsc 70, and hsp 70 expression demonstrated complete agreement in the basal patterns of expression of hsp 27, hsp 60, and hsc 70 between the cultured cells and in situ urothelium (9-11).
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- HSP 17
- Hsp 27
- HSP 84
- HSP 86
- HSP 90
- hsp70 RY