The whole series of 11 studied BAs comprises nonsubstituted (CnA), monosubstituted (lithocholic acid, LCA), disubstituted (deoxycholic acid, DCA, and derivatives chenodeoxycholic, ursodeoxycholic, and hyodeoxycholic acids
, CDCA, UDCA, and HDCA, resp.), and trisubstituted (cholic acid, CA, and dehydroderivatives, 3DHDCA, DHDCA, 3DHCA, and DHCA) cholanic acids.
Abbreviations BA: Bile acid BO: Biliary obstruction CA: Cholic acid CDCA: Chenodeoxycholic acid DCA: Deoxycholic acid FXR: Farnesoid X receptor G: Glucuronide HDCA: Hyodeoxycholic acid HCA: Hyocholic acid LCA: Lithocholic acid LC-MS/MS: Liquid chromatography-tandem mass spectrometry UDPGA: UDP-glucuronic acid UGT: UDP-glucuronosyltransferase MRPs: Multidrug resistance related proteins.
Human liver (black squares, pool of 50 donors) and kidney (white diamonds; pool of 8 donors) microsomes (10 [micro]g) were incubated in the presence of increasing concentrations (1 to 350 [micro]M) of hyodeoxycholic acid (HDCA, (a) and (b)) or hyocholic acid (HCA, (c) and (d)) for 2 hours at 37[degrees]C.