(1982) Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase
The scientists engineered a strain of mice that has 146 extra CAG repeats in a gene that encodes hypoxanthine phosphoribosyltransferase
(HPRT), an enzyme used by all cells.
Doetschman et al., "Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase
gene by homologous recombination in embryonic stem cells," Proceedings of the National Academy of Sciences of the United States of America, vol.
HPRT, hypoxanthine phosphoribosyltransferase
1; *p < 0.05, **p < 0.01; n = 5-8; 1 pg and 10 pg represent 1 [micro]g/kg body weight and 10 [micro]g/kg body weight, respectively.
Expression was normalized against hypoxanthine phosphoribosyltransferase
1 (.HPRT1) and ribosomal protein, large, PO (RPLPO) endogenous expression.
The primer sequences used for StAR, P450scc, 3[beta]-HSD-1, and hypoxanthine phosphoribosyltransferase
(HPRT) (internal control) were based on previously published sequences (Jin et al., 2000; Akingbemi et al., 2003).
A neurologic disorder, LNS is the result of a single mutated gene that codes for a protein known as hypoxanthine phosphoribosyltransferase
The relative intensity was normalized to an endogenous control gene (hypoxanthine phosphoribosyltransferase
MNAzyme RT-qPCR was performed for transcripts of 11 genes of interest (GOIs): ACTB (actin, beta), BCR (breakpoint cluster region), HPRT1 (hypoxanthine phosphoribosyltransferase
1), KRAS, TP53 (tumor protein p53), HMBS (hydroxymethylbilane synthase), PGK1 (phosphoglycerate kinase 1), PMM1 (phosphomannomutase 1), PSMB6 [proteasome (prosome, macropain) subunit, beta type, 6], RPLP0 (ribosomal protein, large, P0), and TFRC.
Regulation of hypoxanthine phosphoribosyltransferase
, glyceraldehydes-3-phosphate dehydrogenase and beta-actin mRNA expression in porcine immune cells and tissues.