Meanwhile, continuous expression of n-NOS with suppression of i-NOS and e-NOS may be an important step in the recurrence process of pterygia.
Orneklerdeki n-NOS, e-NOS ve i-NOS dagilimlari immunperoksidaz yontemi ile degerlendirildi.
Tekrarlayan pteryjium grubunda ise, epitelde n-NOS icin orta siddette Immunreaktivite izlenirken, eNOS hem epitelde hemde bag dokusunda zayif pozitiflik gostermektedir ancak i-NOS immunreaktivitesi getek epitelyum tabakasinda gerek ise bag dokusu tabakasinda maalesef izlenmemektedir.
These data suggest that, (1) macrophages that were incubated in low oxygen tensions produced diminished amounts of i-nos enzyme, resulting in decreased production of NO, or that, (2) there were potentially equal or greater amounts of i-nos produced by these 'hypoxic' macrophages, but due to a lack of dissolved substrate ([O.sub.2]), the enzyme was unable to function.
These data suggest that, (1) exposure to intermittent HBO does increase production of NO by J774 murine macrophages incubated in p[O.sub.2] = 20 or 40mmHg, (2) macrophages that were exposed to lower oxygen tensions may have produced the i-nos enzyme in equal or possibly greater amounts than did those exposed to 95% room air, and that (3) decreased production of NO by the untreated cells was due to a lack of dissolved [O.sub.2] to serve as a cofactor and not necessarily due to a decrease in the production of i-nos.
In conclusion, this study demonstrates that (1) the murine macrophage cell line, J774, produces less nitric oxide when incubated in low p[O.sub.2] conditions, (2) these changes are not necessarily due to a decrease in production of i-nos, rather, that at oxygen tensions that more closely replicate conditions in vivo, dissolved [O.sub.2] may serve as the rate-limiting factor in the enzymatic reaction among i-nos, NADPH and L-arginine, and (3) intermittent exposure to HBO increases production of NO by J774 murine macrophages incubated in p[O.sub.2] = 20mmHg and p[O.sub.2] = 40mmHg.