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Immunofluorescence (IF) staining was operated to detect the TUNELpositive neurons (anti-NeuN, Alexa Fluor[R] 488 conjugated, Millipore, USA, 1: 100) and the immunoreactivity of Iba1 (anti-IBA, ab15690, Abcam, USA, 1: 100, active microglial marker) [21].
Slides were incubated with a primary antibody (IBA1, Abcam, Ab178846 1: 500 diluted with PBS) overnight at 4[degrees]C, washed three times with PBS (pH7.4) for 5 min each, and then incubated with a secondary antibody (secondary antibody: HRP-goat anti-rabbit 1:300) labelled with HRP at room temperature for 50 min.
In our study, we observed the colocalization of TLR2 staining with Iba1 in the cortical penumbra area in the control group, which is in agreement with the results of the abovementioned studies.
Brain sections were incubated with 10% normal goat serum for 1 h at room temperature and then incubated in a humidified chamber with the primary antibodies Iba1 (1:500, Wako Pure Chemical Industries), IL-1[beta] (1:100, R&D Systems Inc.), and TNF-[alpha] (1:50, Proteintech Inc.) overnight at 4[degrees]C.
However, p16 was sparingly expressed in astrocytes labeled with GS, but not in activated microglia when sections were contained with p16 and Iba1 [Figure 2]b.
We analyzed genes for ionized calcium-binding adapter molecule 1 (IBA1), glial fibrillary acidic protein (GFAP), and the 70 kDa heat shock protein (Hsp70).
DeLeo, "A comparison of spinal Iba1 and GFAP expression in rodent models of acute and chronic pain," Brain Research, vol.
Furthermore, immunofluorescence images demonstrated that MST1 colocalized with microglia cells (Iba1) in the right cortex at 72 h after SAH (Figure 2(b)).
This increase of glial cells was not observed in sham animals (Figures 4(b) and 4(d)), and quantification revealed a significantly higher amount of GFAP (p < 0.05) and Iba1 (p < 0.01) reactive cells (Figure 4(e)) in grafted rats.
Kohsaka, "A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage," Biochemical and Biophysical Research Communications, vol.
Representative sections of immunohistochemistry using IBA1 to mark macrophages and microglia are shown (a) and CD3 to mark T cells (b) (bar = 50 [micro]m).