IBDWSIsolation by Distance Web Service
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We tested for isolation by distance by comparing matrices of pairwise genetic distances ([F.sub.ST]) and geographic distances (km) between midpoints of counties in which samples were collected in IBDWS (Jensen et al., 2005).
A pattern of isolation by distance was tested with a Mantel test between minimum geographic distances across the sea between locations (i.e., going around landmasses) against pairwise [F.sub.ST] and Jost' D values with the software IBDWS 3.23 (Jensen et al.
Isolation by distance (IBD) based on genetic differentiation and the logarithm of geographic distance was examined by using 10,000 permutations of the Mantel test implemented in IBDWS v3.15 (http://www.ibdws.sdsu.edu) (Jensen et al.