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One condition was a differentiation protocol where the chemicals of interest were added in the presence of IBMX, insulin, and dexamethasone (MID).
Mouse oocytes were placed in drops of culture media containing 200 [micro]M IBMX for microinjection.
MFME extract treatment in the presence of IBMX has significantly (Pless than 0.
An aliquot of the supernatant was mixed with DMEM containing 1 mmol/L IBMX and 25 mmol/L HEPES and then added to cells stably expressing human GC-A for 3 min at 37 [degrees]C to determine peptide bioactivity.
KEY WORDS: scallop, Chlamys varia, epinephrine, GABA, IBMX, settlement
The increase in cAMP concentration was not caused by the inhibition of PDEs because p,p'-DDT continued to enhance cAMP after inhibition of PDEs by IBMX.
Abbreviations: CT, cryptotanshinone; RA, retinoic acid; Tro, troglitazone; C/EBP, CCAAT/enhancer-binding protein; PPAR[gamma], peroxisome proliferation-activity receptor [gamma]: GAPDH, glycerol-3-phosphate dehydrogenase; IBMX, isobutyl-1-methylxanthine; MTT, 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide; GLUT4, glucose transporter type 4; TNF-[alpha], tumor necrosis factor a: CHOPIO, C/EBP-homologous protein 10; GATA2, GATA-binding protein 2; STAT3, signal transducer and activator of transcription 3; aP2, adipocyte protein 2; MCE, mitotic clonal expansion.
Platelets are stabilized by inclusion of forskolin to increase CAMP concentration and IBMX to inhibit cAMP phosphodiesterase (30, 31).
5 mM IBMX in DMEM with 10% FBS, antibiotics along with different concentrations of SL for only 2 days.
Although this result supports the view that cAMP augmentation and PKA activation are important steps in memory consolidation, an activation of the cGMP-PKG pathway could not be ruled out, since CPT-cAMP also activates cGMP-dependent protein kinase and both IBMX and CPT-cAMP increase cGMP levels.
In addition, 500 [micro]M IBMX was also included in the differentiation media from day 0 to day 4.