ICTVDBInternational Committee on Taxonomy of Viruses Database
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Sequences of 30 nucleotides were aligned using the Bioedit program (version 7.0), with HPV reference sequences obtained from the ICTVdB database (http://www.ictvdb.rothamsted.ac.uk).
A vertebrate viral sequence database (GreeneVrdB) was established by integrating the database of the International Committee on Taxonomy of Viruses (ICTVdB, http://phene.cpmc.columbia.edu), a database that describes viruses at the levels of order, family, genus, and species, and the sequence database of the National Center for Biotechnology Information (NCBI, www.ncbi.nih.gov).
Viral probes were designed to represent a minimum of 3 distinct genomic target regions for every family or genus of vertebrate virus in the ICTVdB. When possible, we chose highly conserved regions within a coding sequence for an enzyme such as a polymerase and 2 other regions that corresponded to more variable structural proteins.