Effects of Mali combined with 5-FU on viability of IEC-6 and Caco-2 cells
Mali (50 [micro]g/mL and 100 [micro]g/mL) significantly decreased viability of IEC-6 and Caco-2 cells compared to cell controls (p < 0.05; Figures 4a and 4b).
Fraxini was the most potent ME on Caco-2 cells; however, it significantly increased the potency of 5-FU chemotherapy on IEC-6 cells compared to Fraxini alone (50 [micro]g/mL).
Another interesting observation was the greater efficacy of Mali on IEC-6 cells compared to Caco-2 cells.
Apart from possessing the lowest I[C.sub.50], Fraxini (50 [micro]g/ mL combined with 5-FU) significantly potentiated 5-FU on IEC-6 cells compared to the same concentration of Fraxini alone.
However, all MEs differentially reduced IEC-6 cell viability.
Grape seed extract protects IEC-6 cells from chemotherapy-induced cytotoxicity and improves parameters of small intestinal mucositis in rats with experimentally-induced mucositis.
Caption: Figure 1: I[C.sub.50] values of Quercus, Fraxini and Mali for IEC-6 and Caco-2 cells.
Caption: Figure 2: Effects of Quercus (1-100 [micro]g/ml) and 5-FU ([micro]M) in combination on viability of IEC-6 (A) and Caco-2 cells (B) after 48hrs.