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Finally, we discovered that the level of multiple IEGs was decreased following treatment with etomidate.
Furthermore, we analyzed levels of oxidative stress, as well as cyclic adenosine 3',5'-monophosphate response element-binding (CREB) protein phosphorylation and immediate early gene (IEG, including Arc, c-fos, and Egr1) expression levels using Western blot analysis.
In summary, we found that etomidate impairs memory by decreasing IEG expression but not by inducing neuronal loss, oxidative stress, or PKA-CREB pathway inhibition.