When we compared the clone size between G-banding and iFISH at initial diagnosis, 16 of 43 (37%) showed differences in clone size of more than 10%.
Correlation of Clone Size Among MDS Subtypes (G-Banding Versus iFISH)
Thirty-five consecutive iFISH tests were performed on 19 patients with aberrant iFISH results.
Patients With Cytogenetic Aberrations Detected Only by iFISH But Not by G-Banding
In 3 of 81 patients (3.7%), cytogenetic abnormalities were detected only by iFISH, but not by G-banding; 5q deletion (case 42) 20q deletion (case 56), and trisomy 8 (case 57) were detected, as presented in Table 3.
Patients With Cytogenetic Aberrations Detected Only by G-Banding But Not by iFISH
Three patients showed cytogenetic aberration only with G-banding, and iFISH failed to detect the corresponding aberrations.
The clone size detected by G-banding and iFISH at initial diagnosis was compared using Kaplan-Meier survival analysis.
IWG Response and Change of iFISH and G-Banding Clone Size
We compared the MDS IWG treatment responses with changes in the iFISH and G-banding clone size at initial diagnosis and follow-up.
Among 28 patients with abnormal iFISH results at initial diagnosis, 26 patients (92.9%) had follow-up G-banding results.
Risk of AML Transformation and Increased Clone Size by iFISH and G-Banding at Follow-up