In this study, we compared the variation in the nucleotide sequences and mRNA expression levels of IGF-1 and IGFBP-1 genes in the yak and lowland cattle to examine whether base mutations or expression levels of genes might be associated with the difference in adaptation to hypoxia.
The identity of IGF-1 and IGFBP-1 gene between yak and other mammals were analyzed using online Multalin software.
A cervical swab was obtained for ph IGFBP-1 measurement with the Actim Partus test.
Swabs were processed for ph IGFBP-1 in all 349 women, but for fetal fibronectin in only 288 of them.
7% decrease in IGFBP-1
and no significant change in IGFBP3 concentration, indicating an increase in the free circulating IGF-1 available to tissues, as compared to 3.
The best-performing glucose tolerance model (fasting glucose, leptin, IGFBP-1, GPT, and Hb[A.
When glycemic markers and insulin were removed from consideration, 5 markers were identified as most informative: IGFBP-1, leptin, PAI-1, GPT, and triglycerides.
The concentrations of IGF-I, IGF-mRNA, IGFBP-3 and IGFBP-1 in the liver and blood were measured via the analytical methods described below.
The prepared sample blots were initially incubated overnight with antibodies against IGPBP-3 and IGFBP-1 at 4[degrees]C, and treated with goat or rat antirabbit horseradish peroxidase.
Nutrient intake and insulin status both determine serum IGFBP-1
concentrations and the extent of phosphorylation of IGFBP-1
, which in turn determines IGF-binding affinity (25).
is present in serum, is the predominant binding protein in amniotic fluid, and is the major IGFBP in fetal and maternal circulation [12, 17-19].
In order to detect the effects of different IGFBP-1
genotypes on production performance traits, we also investigated one population family of Lantang x Landrace (n = 172, gifted by Professor Chen Yaosheng, SCAU, China).