IGFTIndependent Government Flight Test
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Real-time PCR was performed to assess relative transcript levels of Cyp1a1, Cyp1a2, Cyp 1b1, Areg (amphiregulin), Ereg (epiregulin), Egf (epidermal growth factor), RacI (Ras related C3 botulinum substrate l),Tgfb (transforming growth factor beta), Bax (BCL-2 associate X protein), Ccndl (cyclin dl), Mki67 (antigen identified by monoclonal antibody Ki 67), Igft (insulin-like growth factor 1), Fgf2 (fibroblast growth factor 2), Ahr, Egfr (epidermal growth factor receptor), and Esrl (estrogen receptor, alpha) using Assays-on-Demand (Applied Biosystems), with Gusb (glucuronidase, beta) and Actb (actin, beta) as endogenous controls in hepatic and mammary tissues, respectively.