After 2min agitation the colorimetric change was observed at 450 nm (a direct correlation to metabolic activity of biofilms) using a microtiter plate reader (BIO-RAD, iMark
The absorbances for each variable were determined at a wavelength of 450 nm using a microplate reader (iMark
, Bio-Rad, Hercules, CA) against a known standard curve consisting of ER-[alpha] peptide, and concentrations expressed relative to total, cellular-extracted protein concentration.
The protein concentration was detected with a BCA Protein Assay Kit (Beyotime Institute of Biotechnology) using the iMark
Microplate Absorbance Reader (Bio-Rad, CA, USA).
The absorbance was measured against blank at 517 nm via microplate reader (iMark
Microplate Absorbance Reader) [15, 16].
The absorbance was measured at 450 nm using a microplate reader (iMark
; Bio-Rad, Hercules, CA, USA).
The absorbance was read at 570 nm using iMark
Microplate Reader (Bio-Rad, Hercules, CA, USA).
The result was measured using ELISA reader (10013301i, iMark
Microplate Reader at 595 nm).
Then, cells were incubated with CCK-8 for 1 h, and the absorbance at 450 nm was measured with a microplate reader (Bio-Rad iMark
, Hercules, CA).
The plates were measured in an ELISA Reader (iMark
, BioRad) at 450 nm.
Optical density was measured (measured wavelength at 450 nm and reference wavelength at 655 nm) using an iMark
microplate reader (Bio-Rad, Hercules, CA), and the mean background value was subtracted from each value.
Detection is based on the reduction of nitroblue tetrazolium to blue formazan (Song & Hsieh 1994) measured at 650 nm with a microplate reader (Imark
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