IMARKInformation Management Resource Kit
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After 2min agitation the colorimetric change was observed at 450 nm (a direct correlation to metabolic activity of biofilms) using a microtiter plate reader (BIO-RAD, iMark, US).
The absorbances for each variable were determined at a wavelength of 450 nm using a microplate reader (iMark, Bio-Rad, Hercules, CA) against a known standard curve consisting of ER-[alpha] peptide, and concentrations expressed relative to total, cellular-extracted protein concentration.
The protein concentration was detected with a BCA Protein Assay Kit (Beyotime Institute of Biotechnology) using the iMark Microplate Absorbance Reader (Bio-Rad, CA, USA).
The absorbance was measured against blank at 517 nm via microplate reader (iMark Microplate Absorbance Reader) [15, 16].
The absorbance was measured at 450 nm using a microplate reader (iMark; Bio-Rad, Hercules, CA, USA).
The absorbance was read at 570 nm using iMark Microplate Reader (Bio-Rad, Hercules, CA, USA).
The result was measured using ELISA reader (10013301i, iMark Microplate Reader at 595 nm).
Optical density was measured (measured wavelength at 450 nm and reference wavelength at 655 nm) using an iMark microplate reader (Bio-Rad, Hercules, CA), and the mean background value was subtracted from each value.
Detection is based on the reduction of nitroblue tetrazolium to blue formazan (Song & Hsieh 1994) measured at 650 nm with a microplate reader (Imark, BioRad).
IMARK Introducao de um metodo de marketing com mudancas significativas na concepcao do produto ou servico, no posicionamento do produto ou servico, em sua promocao.