Total RNA was extracted from HCT-116 and IMCE cells and tumor-adjacent tissues utilizing the RNeasy mini kit (Qiagen, Carlsbad, CA, USA) and conversely transcribed using the TIANScript reverse transcription kit (TIANGEN Inc.
Lysate of tumors and cellular lysate of IMCE and HCT-116 were prepared by sonication and RIPA buffer.
IMCE and HCT-116 cells were incubated with gradient concentration of silibinin in the absence or presence of LPS for 24 h.
To explore the antitumor activities of silibinin, MTT assay was employed to assess their effects on cell viability in the IMCE cell line and HCT-116 colorectal cancer cells.
Relative expressions of IL-6, IL-1[beta], and TNF-[alpha] in IMCE (a) and HCT-116 cells (b) were presented.
(a, b) IMCE and HCT-116 cells were treated with silibinin at indicated concentrations for 72 h, respectively.
Shinmyozu of the Laboratory of Organic Structural Chemistry, Department of Molecular Chemistry, Graduate School of Sciences & IMCE
, Kyushu University, Japan, for supplying the elemental analysis data of the samples.