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IMViCIndole Methylred Voges-Proskauer Citrate Test
IMViCInternational Motor Vehicle Inspection Committee
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perfringens type D was confirmed through different biochemical tests (IMViC, Sugar fermentation, Catalase reaction, Oxidase tests, Gelatin hydrolysis, Litmus milk reaction and Lecithinase) and PCR.
In IMViC test citrate and methyl red positive, negative to indole and VP.
coli, estas foram isoladas para as provas bioquimicas de indol, vermelho de metila (VM), Voges Proskauer (VP) e citrato (metodo denominado IMViC).
Confirmation of isolates was performed by using classic chemical tests (motility test, ure hydrolysis, acid production from mannitol, production of H2S, IMVIC (Indol, Metil Red, Voges-Proskauer and Citrate), (Lassen, 1975; Krieg and Holt,1984).
Physiological characterization was done by biochemical tests such as staining reaction, oxidase test, catalase test, Imvic tests, amylase production test, gelatin hydrolysis test, urease test, H2S production test, oxidation fermentation test of glucose for carbohydrate utilization, carbohydrate fermentation test etc.
Characteristic colonies with a metallic sheen were transferred to nutrient agar tubes, Gram-stained and subjected to IMViC (Indole, Methyl red test, Voges-Proskauer test, and citrate production) biochemical tests.
Las cepas aisladas fueron caracterizadas e identificadas mediante las pruebas de: tincion de Gram [10], tincion de esporas, alcohol-acido resistente, tension de oxigeno [8] y una serie bioquimica dada por fermentacion de carbohidratos e IMVIC entre otras[11].
Bacterial biochemical tests included carbohydrate fermentation reactions, IMVIC test (Indole, Methyl Red, Voges-Proskauer, Simmon's citrate), catalase test, [H.sub.2]S reducing test, and motility test.
All isolates showed identical reaction to IMViC tests i.e -+-+.