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The study took place at the Instituto de Pesquisa em Aquicultura Ambiental (INPAA), Universidade Estadual do Oeste do Parana, Toledo, Parana, Brasil (24[degrees]46'S, 53[degrees]43'W) from May 2013 to April 2014.
The experiment was conducted in the Laboratory of Reproductive Technology for Farmed Aquatic Animal (LATRAAC), at the Institute for Research in Environmental Aquaculture (InPAA), Western Parana State University (UNIOESTE).