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IP-10IFN-inducible protein-10
IP-10IFN-gamma-inducible protein 10
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FGF-basic, GM-CSF, IP-10, IFN-[gamma], IL-1[alpha], IL-1[beta], IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17, KC, MIP-1-[alpha], MCP-1, MIG, and TNF-[alpha] were evaluated with the mouse cytokine magnetic 20-Plex panel (Novex[R] by Life Technologies, USA) with the immunoassay Luminex Magpix Platform (Luminex Corporation, USA), as previously described (8).
IFN-gamma-inducible protein 10 (IP-10; CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and trafficking.
Finally, treatment-naive tobacco users had significantly higher levels of all biomarkers, except for MMP-9, than nonusers on cART, while tobacco users on cART and nonusers not on cART had variable patterns with MMP-9 higher in the former group and MIG, IL-6, IL-8, IP-10, MCP-1, TNF-[alpha], L selectin, ICAM-1, LBP, and [beta]2-microglobulin higher in the latter group.
The downstream proinflammatory cytokines, including RANTES, MCP-1, and IP-10, also increase in these kidneys.
The concentrations of the following circulation cytokines and chemokines were measured: IL-1[alpha], IL-1[beta], IL-1ra, IL-2, sIL-2R[alpha], IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-15, IL-17a, sCD40L, EGF, Eotaxin, FGF2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFNa2, IFN-[gamma], IP-10, MCP-1, MCP-3/CCL7, MDC/CCL22, MIP-1a, MIP-1[beta], TGF[alpha], TNF[alpha], TNF[beta], and VEGF.
The concentrations of CXCL9, IP-10, CXCL11, IL-8, IL-10, and CCL2 in the serum were significantly higher in the groups of DENV-infected patients during the first two weeks than the control group (P < 0.05).
Our data showed that the median concentrations of CXCL10 (IP-10), CCL11 (eotaxin), CCL17 (TARC), and CCL4 (MIP-1) were significantly elevated in the BALF of asthmatics compared with controls, with increases accompanying disease severity.
Elevated levels of RANTES (and IP-10 initially) indicate T-cell activation, possibly reflecting ongoing viral replication in the joints, also as described in chikungunya (6).
After this period, the supernatant was collected and nine cytokines and chemokines released (IL-1[beta], IL-6, IL-10, IL-12, IFN-[alpha]2, TFN-[alpha], IP-10, MCP-1 and RANTES) were quantified using a multiplex assay (HCYTOMAG-60 K, Milliplex[R] kit, Merck Millipore, Germany) with Luminex[R] Magpix[TM] technology (Austin, TX, USA).
The concentrations of interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL10), interleukin-12 (IL-12), granulocyte-colony stimulating factor (G-CSF), interferon-gamma (INF-[gamma]), tumor necrosis factor-[alpha] (TNF-[alpha]), monocyte chemoattractant protein-1 (MCP-1) (CCL-2), CXCL10 chemokine (IP-10), plateletderived growth factor (PDGF), basic-fibroblastic growth factor (bFGF), and vascular endothelial growth factor (VEGF) are reported in Figure 1 for ASCs, MG-63, and SaOs-2 cells that were kept both under maintaining and differentiation media.
Enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the levels of IL-8, CCL20, IP-10, and GRO[alpha] (R&D Systems, Minneapolis, MN, USA) in cell culture supernatants.