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In addition, a study in murine stem cells showed that the FM550 components TPP and IPTP divert osteogenesis to the adipogenesis pathway through activation of peroxisome proliferatoractivated receptor [gamma] (PPAR[gamma]) (Pillai et al.
We show that FM550 and its two major components, TPP and IPTP, induce adipogenesis in human primary cells.
Isopropylated triphenyl phosphate (IPTP) was a generous gift from W.
Treatments with the chemicals of interest were performed as follows: a) when the chemicals were tested for their ability to replace troglitazone, the cells were treated with dexamethasone from day 0 and the test chemical (0-200 [micro]M FM550, 0-200 [micro]M IPTP, 0-20 [micro]M TPP, 0-20 [micro]M TBPH, 0-20 [micro]M TBB) from day 2, with media replacements on days 4 and 8 (MID condition); b) when the chemicals were to replace dexamethasone, the test chemical was added from day 0, and troglitazone with the test chemical were added from day 2 onward and replaced with media changes on days 2, 4, and 8 (MIT condition).
Total RNA from preadipocytes from five donors treated with MID, MITD, TPP, or IPTP was collected on day 6 of differentiation of preadipocytes, from five donors were used for RNA-Seq.
Effects of FM550, IPTP, and TPP on Adipogenesis in Human Primary Preadipocytes
IPTP treatment induced a significant ~ 4-fold increase in lipid accumulation by Nile red staining at both 10 and 20 [micro]M, as compared with the MID condition (Figure 1C).
Effects of FM550, IPTP, and TPP on FABP4 Protein Expression in the Presence of Dexamethasone
Mazieres, "Rateless codes and big downloads," in Proceedings of the 2nd International Workshop Peer-to-Peer Systems (IPTPS 03), February 2003.
Rodriguez, "Anatomy of a p2p content distribution system with network coding," in Proceedings of the 5th International Workshop on Peer-to-Peer Systems (IPTPS 06), February 2006.
In IPTPS 'Oh Revised Papers from the First International Workshop on Peer-to-Peer Systems, pages 251-260, London, UK, 2002.
Meo, "A detailed measurement of skype network traffic," in Proceedings of the 7th International Conference on Peer-to-Peer Systems (IPTPS '08), p.
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