IROMPIron-Regulated Outer Membrane Protein
IROMPIron-Repressed Outer Membrane Protein
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Extraction of IROMPs: IROMPs were characterized according to a modified method of Goel and Kapil (20).
Bactericidal activity: Total viable count was performed to demonstrate bactericidal activity of polyclonal antibodies raised against IROMPs according to the modified method described by Poolman et al (29).
S26 was seen to overexpress bands corresponding to IROMPs in comparison to the isolate which was grown in the presence of iron in the medium (Fig.
The polyclonal antibodies raised against IROMPs in rabbits showed bactericidal activity against the organism tested and specifically killed the bacteria grown in iron deficit medium in a dose dependant manner (Fig.