Differential regulation of IRP1 and IRP2 by nitric oxide in rat hepatoma cells.
Caracteristicas de las principales moleculas que intervienen en el metabolismo del hierro Proteina Sintesis Funcion IRP1 Higado Placenta Rinones Proteina reguladora de hierro con actividad aconitasa.
Both IRPs are homologous to the mitochondrial TCA cycle enzyme aconitase that converts citrate to isocitrate using an iron sulfur cluster (4Fe-4S) as a cofactor, but only IRP1 can assemble a cluster when sufficient iron is available, thus functioning as cytosolic aconitase, which is the prevailing form in most cells.
Ablation of both IRP1 and IRP2, which are ubiquitously expressed, is early embryonic lethal whereas single knockout mice are viable, indicating that the two IRPs can compensate for each other and confirming the in vitro data which suggested essential but largely overlapping functions.
Despite reports showing IRP1 activation in cells exposed to [H.
We also showed that anthracycline cardiotoxicity is related to ROS-dependent and ROS-independent disruption of cardiac iron homeostasis due to targeted interaction of DOX with IRP1, which leads to a "null" IRP1 devoid of both its functions and hence it is unable to sense iron levels and to regulate iron homeostasis.
37) No obstante, cuando disminuye la actividad de la IRP1
se incrementan las concentraciones de ferritina y de ferroportina, y por tanto aumenta la reserva de hierro en la placenta y, en forma moderada, el transporte de hierro al feto.
The new information solidified the team's hunch that the particular region where IRP1 binds to the messenger RNA is a potential drug target.
With other research teams, we are investigating novel therapies that remove excessive iron, and we're looking at the precise spot on the messenger RNA where IRP1 binds to screen for drugs that specifically prevent APP production," said Dr.
During low-iron conditions, synthesis of both IRP1 and IRP2 increases.
Iron sulfur clusters have also been shown in cytoplasm within IRP1 and in the nucleus within an enzyme that is involved in base excision repair.
GLRX5 deficiency causes impaired ISC synthesis that activates cytosolic IRP1 and finally leads to increased cellular iron import.